咖啡因和代谢物分析

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咖啡因和代谢物分析 Caffeine and Metabolites
        Conditions
Column: ACE 5 SuperC18
Dimensions: 150 x 4.6 mm
Part Number: EXL-1211-1546U
Mobile Phase:
        A: 20 mM ammonium acetate pH 7.0 in H2O
B: 20 mM ammonium acetate pH 7.0 in MeCN/H2O (90:10 v/v)

Time(mins)	%B
0	2
45	15
48	15
49	2

        Post time 10 minutes
        Flow Rate: 1 mL/min
Injection: 1 μL
Temperature: 60 °C
Detection: UV, 273 nm