饮料中的磷酸在HPLC柱混合型色谱柱上的定量分析
App ID: FLM-C210058
Equipment
Agilent 1100 HPLC system with Sedere 75 ELSD Analytical Balance
HPLC Column: Comixsil HAX 3 um 3.0x100 mm(P/N: FMD-HAX3-YONU)
Materials:
Soft drinks (Coke and Pepsi products) ACN (Tedia, catalog #AS1127)
Distilled water (Aldrich, catalog #270733) Ammonium acetate (Aldrich, catalog #7262) Acetic acid (Aldrich, catalog #6283) Phosphoric acid (Aldrich, catalog #345245)
Method Description
HPLC Pump set up: A: DI water, B: Acetonitrile, C: 100 mM
Ammonium acetate pH 4
Mobile phase delivery: A: 30%, B: 60%, C: 10%
Column: Comixsil HAX 3 um 3.0x100 mm
Flow: 0.5 ml/min
Detection: ELSD. 40oC
Sample: Standards and soft drinks
饮料中的磷酸在HPLC柱混合型色谱柱上的定量分析
|
Concentration, mg/ml |
Peak Area Average, mAU*s |
Peak Area, Inj. 1, mAU*s |
Peak Area, Inj. 2, mAU*s |
Peak Area, Inj. 3, mAU*s |
Concnetration of Phosphoric Acid, mg/ml |
|
0.2 |
97530 |
97770 |
98943 |
95876 |
- |
|
0.5 |
412588 |
412190 |
413682 |
411892 |
- |
|
1 |
1194435 |
1189859 |
1198924 |
1194521 |
- |
|
2 |
3182351 |
3172520 |
3186903 |
3187630 |
- |
|
Pepsi Zero |
424361 |
424239 |
424658 |
424187 |
0.47 |
|
Pepsi Black Cherry |
431486 |
431375 |
431657 |
431425 |
0.47 |
|
Pepsi Diet Wild Cherry |
634614 |
634305 |
634856 |
634682 |
0.59 |
|
Coca Cola Zero |
675667 |
680207 |
670911 |
675882 |
0.61 |
|
Coca Cola Diet |
323945 |
323873 |
324104 |
323857 |
0.41 |
|
Coca Cola |
633553 |
633878 |
632119 |
634663 |
0.59 |
