App ID: FLM-C210057
果汁中的维生素C在Comixsil HAX混合型柱上的HPLC定量分析
Equipment
Agilent 1100 HPLC system with DAD Analytical Balance
HPLC Column: Comixsil HAX 3 um 3.0x100 mm(P/N: FMD-HAX3-YONU)
Materials:
Fruit juices
ACN (Tedia, catalog #AS1127)
Distilled water (Aldrich, catalog #270733) Ammonium acetate (Aldrich, catalog #7262) Acetic acid (Aldrich, catalog #6283)
Vitamin C (Aldrich, catalog #A92902)
Method Description 2
HPLC Pump set up: A: DI water, B: Acetonitrile, C: 100 mM Ammonium acetate pH 4
Mobile phase composition: : A: 30%, B: 60%, C: 10%
Vitamin C Calibration and Sample Analysis
Column: Comixsil HAX 3 um 3.0x100 mm
Flow: 0.5 ml/min
Detection: 275 nm
Sample: Standards and juices
本方法同样适合质谱检测器
